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rat antimouse marco ed31  (Bio-Rad)


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    Bio-Rad rat antimouse marco ed31
    Figure 2. (A, B) Wildtype and <t>Marco−/−macrophages</t> were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO <t>ED31</t> mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the
    Rat Antimouse Marco Ed31, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+antimouse+marco+ed31/Rat+anti+Mouse+MARCO/pm38494423-128-34-41
    Average 93 stars, based on 138 article reviews
    rat antimouse marco ed31 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages."

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.

    Journal: European journal of immunology

    doi: 10.1002/eji.202350771

    Figure 2. (A, B) Wildtype and Marco−/−macrophages were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO ED31 mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the
    Figure Legend Snippet: Figure 2. (A, B) Wildtype and Marco−/−macrophages were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO ED31 mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the

    Techniques Used: Infection

    Related Articles

    Infection:

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.
    Article Snippet: .. Meanwhile, to follow infection from uptake through to vomocytosis, timelapse imaging began immediately following macrophage incubation with C. neoformans at an MOI 0.5:1.Where applicable, macrophages were pre-treated with 400 μg/mL polyG (Sigma-Aldrich; cat#: P4404), rat antimouse MARCO ED31 clone monoclonal antibody (BioRad; Cat#: MCA1849), or anti-rat IgG1 isotype control (Invitrogen; cat#: 14430182) for 30 min at 37°C prior to 2 h infected with nonopsonised C. neoformans. ..

    Imaging:

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.
    Article Snippet: .. Meanwhile, to follow infection from uptake through to vomocytosis, timelapse imaging began immediately following macrophage incubation with C. neoformans at an MOI 0.5:1.Where applicable, macrophages were pre-treated with 400 μg/mL polyG (Sigma-Aldrich; cat#: P4404), rat antimouse MARCO ED31 clone monoclonal antibody (BioRad; Cat#: MCA1849), or anti-rat IgG1 isotype control (Invitrogen; cat#: 14430182) for 30 min at 37°C prior to 2 h infected with nonopsonised C. neoformans. ..

    Incubation:

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.
    Article Snippet: .. Meanwhile, to follow infection from uptake through to vomocytosis, timelapse imaging began immediately following macrophage incubation with C. neoformans at an MOI 0.5:1.Where applicable, macrophages were pre-treated with 400 μg/mL polyG (Sigma-Aldrich; cat#: P4404), rat antimouse MARCO ED31 clone monoclonal antibody (BioRad; Cat#: MCA1849), or anti-rat IgG1 isotype control (Invitrogen; cat#: 14430182) for 30 min at 37°C prior to 2 h infected with nonopsonised C. neoformans. ..

    Control:

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.
    Article Snippet: .. Meanwhile, to follow infection from uptake through to vomocytosis, timelapse imaging began immediately following macrophage incubation with C. neoformans at an MOI 0.5:1.Where applicable, macrophages were pre-treated with 400 μg/mL polyG (Sigma-Aldrich; cat#: P4404), rat antimouse MARCO ED31 clone monoclonal antibody (BioRad; Cat#: MCA1849), or anti-rat IgG1 isotype control (Invitrogen; cat#: 14430182) for 30 min at 37°C prior to 2 h infected with nonopsonised C. neoformans. ..



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    rat antimouse marco ed31  (Bio-Rad)
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    Bio-Rad rat antimouse marco ed31
    Figure 2. (A, B) Wildtype and <t>Marco−/−macrophages</t> were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO <t>ED31</t> mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the
    Rat Antimouse Marco Ed31, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+antimouse+marco+ed31/Rat+anti+Mouse+MARCO/pm38494423-128-34-41
    Average 93 stars, based on 1 article reviews
    rat antimouse marco ed31 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. (A, B) Wildtype and Marco−/−macrophages were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO ED31 mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the

    Journal: European journal of immunology

    Article Title: Loss of the scavenger receptor MARCO results in uncontrolled vomocytosis of fungi from macrophages.

    doi: 10.1002/eji.202350771

    Figure Lengend Snippet: Figure 2. (A, B) Wildtype and Marco−/−macrophages were stimulated with 10 ng/mL LPS overnight, then infected with anti-GXM 18B7 antibody opsonised C. neoformans. After 2 h infection, images were acquired every 5 min for 16 h. (B) Wildtype: n = 27 vomocytosis events out of 220 infected macrophages; Marco−/−: n = 153 vomocytosis events out of 166 infected macrophages. (C, D) LPS-stimulated macrophages were infected with a yeast- locked TetO-NRG1 C. albicans strain. Images were acquired every 5 min for 6 h. The phagocytic index (%) represents the percentage of macrophages that phagocytosed one or more fungal cells. At least 200 macrophages were observed per condition. Vomocytosis (%) is the percentage of infected macrophages that experienced at least one expulsion event. (D) Wildtype: n = 4 vomocytosis events out of 112 infected macrophages; Marco−/−: n = 36 vomocytosis events out of 101 infected macrophages. (E) Representative image showing vomocytosis of C. albicans from Marco−/−MPI cells. Time is presented in hh:mm:ss; red arrows follow the course of a vomocytosis event; scale bar = 10 μm. (F, G) Wildtype macrophages were stim- ulated overnight with 10 ng/mL LPS. The following day, cells were pre-treated with polyguanylic acid (polyG) or (H, I) anti-MARCO ED31 mono- clonal antibody (mAb) for 30 min then infected with non-opsonised C. neoformans still in the presence of polyG or anti-MARCO mAb. Images were acquired every 5 min for 16 h. Phagocytosis was quantified as the total number of internalised fungi per 100 macrophages at the beginning of the

    Article Snippet: Meanwhile, to follow infection from uptake through to vomocytosis, timelapse imaging began immediately following macrophage incubation with C. neoformans at an MOI 0.5:1.Where applicable, macrophages were pre-treated with 400 μg/mL polyG (Sigma-Aldrich; cat#: P4404), rat antimouse MARCO ED31 clone monoclonal antibody (BioRad; Cat#: MCA1849), or anti-rat IgG1 isotype control (Invitrogen; cat#: 14430182) for 30 min at 37°C prior to 2 h infected with nonopsonised C. neoformans.

    Techniques: Infection